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KMID : 0900920010250020101
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Korean journal of Animal Reproduction
2001 Volume.25 No. 2 p.101 ~ p.111
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Effects of FSH and LH on Maturation of Bovine Preantral Follicle
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Kim D.-J.
Chung H.-J.
Kim Dong-Hoon
Uhm S.-J.
Lee Hoon-Taek
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Abstract
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The present study was conducted to develop an in vitro culture system that would support bovine follicle growth from preantral to antral stage, oocyte maturation, fertilization, and embryonic development. Bovine preantral follicles (150pm1.2 {mu}{textrm}{m}) surrounded by theca cell were isolated ezymatically and mechanically from ovarian cortical slides in Leibovitz L-l5 medium containing 1 mg/mell collagenase and 0.2 mg/mell DNase I and cultured for 25 days in the presence of different concentrations of bovine FSH and LH in alphaMEM medium. The survival and growth rates of follicles cultured in the presence of FSH (10~150 ng/mell) were significantly higher than those of control group (P ＜ 0.001), but no significant differences were observed in survival and growth rates of follicles between the LH treatment groups (1~125 ng/mell) and the control. The survival (40%) and growth (244 pm 0.5 mutextrm{g}) of follicles cultured with FSH (90 ng/mell) and LH (25 ng/mell) were higher than those of control (25%, 160 pm1.0 mutextrm{g}). Finally, 50% percent of healthy antral follicles were obtained, and almost 60% of them has complete meiotic division with 1st polar body (18.1%) and 10.0% have developed to the cleaved embryo and blastocyst stage. These results suggest that bovine preantral follicle with intact theca cell can grow to the antral stage using these culture conditions, and that oocytes from in vitro-matured bovine preantral follicle may acquire meiotic competence and can undergo fertilization and development.
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KEYWORD
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